Applying watershed algorithms to the segmentation of clustered nuclei

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Applying watershed algorithms to the segmentation of clustered nuclei.

Cluster division is a critical issue in fluorescence microscopy-based analytical cytology when preparation protocols do not provide appropriate separation of objects. Overlooking clustered nuclei and analyzing only isolated nuclei may dramatically increase analysis time or affect the statistical validation of the results. Automatic segmentation of clustered nuclei requires the implementation of...

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ژورنال

عنوان ژورنال: Cytometry

سال: 1998

ISSN: 0196-4763

DOI: 10.1002/(sici)1097-0320(19970801)28:4<289::aid-cyto3>3.0.co;2-7